OPTIMIZATION OF ITRAQ LABELLING COUPLED TO OFFGEL FRACTIONATION AS A PROTEOMIC WORKFLOW TO THE ANALYSIS OF MICROSOMAL PROTEINS OF MEDICAGO TRUNCATULA ROOTS

Abstract Background Shotgun proteomics represents an attractive technical framework for the study of membrane proteins that are generally difficult to resolve using two-dimensional gel electrophoresis.The use of iTRAQ, a set of amine-specific isobaric tags, is currently the labelling method of choice allowing multiplexing of up to eight samples and

Kendenglembu Glenmore Farm is a cocoa producing area in Banyuwangi.This plantation is managed by BUMN, namely PTPN XII.One of the results of cocoa processing is in the form of cocoa powder which is marketed through a variety of foods and beverages.These various foods and beverages are partly managed through IIK Kendneglemembu, hereinafter referred

The mantle transition zone (MTZ) plays a key role in the deep global material cycle, while the water content in MTZ is debated from saturated to dry.Since the electrical conductivity is highly sensitive to water, its accurate estimation will greatly help Hairball Remedy reveal the water content.The high quality and plenty of data are crucial for gl

Polymer concrete is a composite material realized with resin and Medicated Oils aggregates.In the present study the epoxy resin was used for binding the aggregates.In the composition were introduced near the fly ash, used as filler, the cellulose fibers.The mechanical characteristics such as compressive strength, flexural strength and split tensile

Many organisms exhibit temporal rhythms in gene expression that propel diurnal cycles in physiology.In the liver of mammals, these rhythms are controlled by transcription-translation feedback loops of the core circadian clock and by feeding-fasting cycles.To better understand the regulatory interplay between the circadian clock and feeding rhythms,